Poster – In Situ Multiplex Analysis Of Resident Microglia And Infiltrating Macrophages In Glioblastoma

Glioblastoma multiforme (GBM) is the most malignant primary brain tumor. Resident microglia and peripheral infiltrating macrophages account for up to half of the non-neoplastic cells in a GBM. These tumor-associated macrophages have been implicated in proliferation, angiogenesis, and immunosuppression in GBM and can influence the efficacy of chemo-, radio-, and immunotherapies.

However, certain cancer specific interactions have been associated with either microglia or macrophages, necessitating an approach that can delineate the two populations. Multiplex immunofluorescence offers a technical advantage that allows for the profound phenotyping of cells in the tumor microenvironment as well as their spatial organization.

In this poster we show you how we designed a multiplex immunofluorescence protocol to differentiate microglia and infiltrating M1/M2 macrophages in GBM in situ.