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Custom Flow Cytometry Assay Development

From cellular subtype identification to immunophenotyping to measure drug occupancy of cellular target markers, Cerba Research offers tailor-made assay development dedicated to the objectives of your clinical trial.

From panel design to validation, give your custom flow cytometry assays a fit-for-purpose approach with regular conversation throughout your project.

From panel design to validation, give your assay a fit for purpose approach with regular conversation throughout your project

Panel Design

● Ab selection (clone) ● Fluorochrome selection


● With the same matrix as for the clinical trial ● Test the staining protocol instrument setup ● Compensation ● And more


● Identify the populations of interest ● Assess the need for controls (FMO or Isotype) ● Define gating sequence for final reporting


● Inter/Intra assay precision ● Inter-operator and inter-instrument ● Sample stability ● Additional parameters (e.g post stained stability, sensitivity (LLOQ))

Custom Flow Cytometry Assays

With a customized flow cytometry assay development and validation approach, Cerba Research utilizes the appropriate sample types for your project.


A scientist standing in a lab whilst testing samples
a scientist in a lab operating a BD FACASDuet sample preparation system

The Importance of Validation

All biomarkers must undergo advanced validation to ensure data is interpretable within multiple time points, within large patient cohorts, and tested at different locations around the world.

All the markers used during your study undergo advanced validation to guarantee the interpretation of data

Flow Cytometry Assay Specimens

Cerba Research is able to perform your flow cytometry assay with whole blood, bone marrow aspirate or PBMCs.

In this video, Cerba Research takes you on an in-depth tour of their CerbACT immuno-oncology center of excellence in Asia.
5 scientists standing in a circle smiling and looking down at the camera. They have all put their hands together in a huddle


Our custom flow cytometry assays are harmonized with global SOPs and standardized instrument settings for immunophenotyping and fluorescence quantification.

Cerba US Expansion Tour VF.00_04_27_19.Still010

Reach out to our flow cytometry team and discover how we can help advance your research

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