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DNA and RNA Extraction

Our DNA and RNA Extraction Services

Cerba Research can propose a wide array of genomic services, including DNA and RNA extraction, thanks to a full range of automated and harmonized protocols available.

gDNA Isolation

For gDNA isolation from whole blood, we normally extract DNA by using the QIAamp DNA blood Mini/Midi/Maxi kits.This extraction can be performed on different types of matrix including whole blood, FFPE tissue, plasma, body fluids, lymphocytes and more.

RNA Isolation

For RNA, the procedure is complicated by the ubiquitous presence of ribonuclease enzymes that rapidly degrade RNA. Several methods are used to stabilize and isolate RNA from samples, such as PAXgen® Blood RNA tubes (intended for immediate stabilization of intracellular RNA from Qiagen) or RNAlater Preserved Tissues (long-term sample preservation used mostly for biopsies).

Thanks to a fully automated platform available in most of our laboratories, Cerba Research can ensure a quick turnaround time for your nucleic acid extraction needs, with a full sample traceability.

 

A photo of a scientist picking up a test tube vial full of blood out of a test tube rack

DNA and RNA Extraction From a Range of Biospecimens

Our experience over the years allows us to respond to your nucleic acid extraction needs from the following biospecimens:

  • Body fluids (urine, feces, scrapes/smears/swabs in collection medium)
  • Bone marrow aspirates
  • Buffy coat
  • Core needle biopsies
  • FFPE tissue
  • Fine needle aspirates
  • Flash frozen biopsies
  • PBMCs
  • Plasma/serum (could be frozen EDTA plasma)
  • Punch biopsies
  • Whole blood

 

Thanks to a full range of instruments for automated workflow:

  • Promega MaxPrep
  • Promega Maxwell RCS 48
  • Perkin Elmer Janus G3
  • Perkin Elmer Chemagic

Designed to process small numbers of samples, these manual genomic DNA extraction kits offer several methods for DNA purification, such as:

  • Solution-based
  • Silica membrane-based
  • Resin-based
  • Magnetic options

DNA and RNA extraction are automated with Maxwell® HT systems, which are customizable to meet the needs of your laboratory. Based on convenient cartridges for specific sample types, Maxwell® RSC 48 provides automated purification from 1-48 samples.

 

 

Reach out to our genomics team and discover how we can help advance your research in the field of DNA/RNA Extraction