As flow cytometry is a powerful tool to characterize cellular populations, it is critical to have standardized instruments within and across different labs and/or regions for global clinical trials. The Cytometer Setup & QC software in the BD FACSLyric™ instrument should correct for daily fluctuations within one instrument and across instruments using Bright Bead Median Target Values (BBMV).
To assess the capability of the software module to standardize flow cytometry assays, we evaluated the Median Fluorescence Intensity (MFI) between instruments and within instruments over time, using both BD® Cytometer Setup and Tracking (CS&T) beads (BD Biosciences) and SPHERO™ Ultra Rainbow calibration particles (Spherotech).
Poster – Comparison of BD FACSLyric™ Instrument Performance in a Global Setting
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