Next generation sequencing (NGS) allows for the simultaneous analysis of the gene’s coding for the 16S ribosomal RNA for bacterial identification and the ITS (internal Transcribe Spacer) region for the characterization of fungal species. This is possible due to hundreds of thousands of copies of DNA sequences. 

The obtained sequences are compared with well-known sequences of bacterial or fungal genes detailed in the Silva algorithm (97%.)

This novel test is performed on stool samples and allows the mapping of the intestinal bacterial and fungal flora including the species that cannot be cultivated.